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peg tethers  (Tokyo Chemical Industry)

 
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    Structured Review

    Tokyo Chemical Industry peg tethers
    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa <t>(PEG_0.8k),</t> 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa <t>(20-bp</t> <t>dsDNA),</t> 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.
    Peg Tethers, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peg+tethers/pmc12664379-65-30-35?v=Tokyo+Chemical+Industry
    Average 86 stars, based on 1 article reviews
    peg tethers - by Bioz Stars, 2026-08
    86/100 stars

    Images

    1) Product Images from "In vitro motility-based tether-scanning of the kinesin motor domain"

    Article Title: In vitro motility-based tether-scanning of the kinesin motor domain

    Journal: MethodsX

    doi: 10.1016/j.mex.2025.103719

    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.
    Figure Legend Snippet: SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.

    Techniques Used: SDS Page, Construct

    In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.
    Figure Legend Snippet: In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.

    Techniques Used: In Vitro, Gliding Assay, Avidin-Biotin Assay, Conjugation Assay



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    Image Search Results


    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.

    Journal: MethodsX

    Article Title: In vitro motility-based tether-scanning of the kinesin motor domain

    doi: 10.1016/j.mex.2025.103719

    Figure Lengend Snippet: SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.

    Article Snippet: The specific DNA sequences used were as follows: 20-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCT-biotin-3′ 40-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 60-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 20-bp dsDNA: 5′-amine-TACTAACTAATCGTCCAACG-3′ 5′-biotin-CGTTGGACGATTAGTTAGTA-3′ 40-bp dsDNA: 5′-amine-CGTTGGACACGACAGGTCCTTAGACTGAAATTAGTTAGTA-3′ 5′-biotin-TACTAACTAATTTCAGTCTAAGGACCTGTCGTGTCCAACG-3′ 60-bp dsDNA: 5′-amine-ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGAGCCATCTGCATGAATGAATCGGCCAACG-3′ 5′-biotin-CGTTGGCCGATTCATTCATGCAGATGGCTCGACAGGTTTCCCGACTGGAAAGCGGGCAGT-3′ As PEG tethers, Biotin-PEG11-amine (0.8 kDa, Tokyo Chemical Industry, Tokyo, Japan), Biotin-PEG2k-Maleimide (2 kDa, Biopharma PEG Scientific), and Biotin-PEG5k-Maleimide (5 kDa, Laysan Bio) were purchased.

    Techniques: SDS Page, Construct

    In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.

    Journal: MethodsX

    Article Title: In vitro motility-based tether-scanning of the kinesin motor domain

    doi: 10.1016/j.mex.2025.103719

    Figure Lengend Snippet: In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.

    Article Snippet: The specific DNA sequences used were as follows: 20-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCT-biotin-3′ 40-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 60-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 20-bp dsDNA: 5′-amine-TACTAACTAATCGTCCAACG-3′ 5′-biotin-CGTTGGACGATTAGTTAGTA-3′ 40-bp dsDNA: 5′-amine-CGTTGGACACGACAGGTCCTTAGACTGAAATTAGTTAGTA-3′ 5′-biotin-TACTAACTAATTTCAGTCTAAGGACCTGTCGTGTCCAACG-3′ 60-bp dsDNA: 5′-amine-ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGAGCCATCTGCATGAATGAATCGGCCAACG-3′ 5′-biotin-CGTTGGCCGATTCATTCATGCAGATGGCTCGACAGGTTTCCCGACTGGAAAGCGGGCAGT-3′ As PEG tethers, Biotin-PEG11-amine (0.8 kDa, Tokyo Chemical Industry, Tokyo, Japan), Biotin-PEG2k-Maleimide (2 kDa, Biopharma PEG Scientific), and Biotin-PEG5k-Maleimide (5 kDa, Laysan Bio) were purchased.

    Techniques: In Vitro, Gliding Assay, Avidin-Biotin Assay, Conjugation Assay